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dc.creatorTolstano, Axel A
dc.creatorVillarreal C, José Luis
dc.creatorTorres J, Franklin E.
dc.creatorLozano H., Edward
dc.creatorMovilla P, Nelys P.
dc.creatorBarroso M, Lina M
dc.descriptionIntroduction: Diseases of the circulatory system represent one of the greatest public health problems worldwide, nationally and regionally. The pathogenic mechanism that underlies this pathology is atherosclerosis. There are several factors that favor the etiopathogeny of the atherosclerotic lesion. Infections play an important role. Infection with Herpes Simplex Virus has been considered as an emerging risk factor. Objective: To perform molecular diagnosis of infection by Herpes Simplex virus type 1 and type 2 in human atherosclerotic tissue. Method: Viral DNA extraction was performed from atheromas using the commercial PureGenomeTM Tissue DNA Extraction kit. The amplification of the viral genetic material was performed by real-time PCR (qPCR) with the commercial kit "Human Herpes Virus 2 (Herpes simplex type 2) UL36 region genesig Standard Kit and Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene Genesig Standard Kit ". Results: A total of 102 samples of atheromas were obtained, extracted from different anatomical sources. Three samples were positive for HSV type 1 (3/102). No sample showed genetic material for HSV type 2 (0/102). Conclusion: The etiopathogenesis of atherosclerosis is a highly complex process. Viruses play an important role, especially the infection by Herpes simplex virus type 1. The infection by this virus generates changes at the level of vascular and non-vascular cells, favoring the accumulation of chemically oxidized low density lipoproteins, important for the atherogenesisen-US
dc.descriptionIntroducción: Las enfermedades del sistema circulatorio representan uno de los mayores problemas de salud pública a nivel mundial, nacional y regional. El mecanismo patogénico que subyace esta patología es la aterosclerosis. Existen varios factores que favorecen la etiopatogenia de la lesión aterosclerótica. Las infecciones, juegan un papel importante. La infección por el Virus del Herpes Simplex se ha considerado como un factor de riesgo emergente. Objetivo: Realizar diagnóstico molecular de infección por Virus Herpes Simplex tipo 1 y tipo 2 en tejido aterosclerótico humano. Método: Se realizó extracción de ADN viral a partir de ateromas usando el kit comercial PureGenomeTM Tissue DNA Extraction. La amplificación del material genético viral se realizó por PCR en tiempo real (qPCR) con el kit comercial “Human Herpes Virus 2 (Herpes simplex type 2) UL36 region genesig Standard Kit y Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene. Genesig Standard Kit”. Resultados: En total se obtuvieron 102 muestras de ateromas, extraídas de diferentes fuentes anatómicas. Tres muestras resultaron positivas para VHS tipo 1 (3/102). Ninguna muestra evidenció material genético para VHS tipo 2 (0/102). Conclusión: La etiopatogenia de la aterosclerosis es un proceso altamente complejo. Los virus juegan un papel importante, en especial la infección por Virus del herpes simplex tipo 1. La infección por este virus genera cambios a nivel de las células vasculares y no vasculares, favoreciendo el acumulo de lipoproteínas de baja densidad químicamente oxidadas, importantes para la aterogé
dc.publisherUniversidad Librees-ES
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dc.rightsDerechos de autor 2018 Biocienciases-ES
dc.sourceBiociencias; Vol 13 No 2 (2018): Biociencias; 85-93en-US
dc.sourceBiociencias; Vol. 13 Núm. 2 (2018): Biociencias; 85-93es-ES
dc.subjectEnfermedades circulatoriases-ES
dc.subjectVirus Herpes simplexes-ES
dc.rights.licenseAtribución-NoComercial-SinDerivadas 2.5 Colombia*

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